WT1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB1621
- Bilder (6)
| Artikelname: | WT1 Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB1621 |
| Hersteller Artikelnummer: | orb1621 |
| Alternativnummer: | BYT-ORB1621-50,BYT-ORB1621-100,BYT-ORB1621-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | FC, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse |
| Immunogen: | KLH conjugated synthetic peptide derived from human WT1 (301-400/449aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | AWT1, GUD, NPHS4, WAGR, WIT-2, WT-1, WT33, D630046I19Rik, WT1_HUMAN, WT1, WT1_MOUSE, WT1_RAT, |
| WT1 Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 55 kDa |
| UniProt: | P19544 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | WT1 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/test |
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Blank control: Molt-4. Primary Antibody (green line): Rabbit Anti-Wilms Tumor Protein antibody (orb1621), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Wilms Tumor Protein) Polyclonal Antibody, Unconjugated (orb1621) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Wilms Tumor Protein) Polyclonal Antibody, Unconjugated (orb1621) at 1:400 overnight at 4C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb868805) for 90 minutes, and DAPI for nuclei staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Wilms Tumor Protein) Polyclonal Antibody, Unconjugated (orb1621) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Mouse Ovary tissue lysates, Lane 3: Mouse Liver tissue lysates, Lane 4: Human HL-60 cell lysates, Primary: Anti-WT1 (orb1621) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kDa, Observed band size: 50 kDa. |
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Sample: TM4 Cell (Mouse) Lysate at 40 ug, Primary: Anti-Wilms Tumor Protein (orb1621) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kD, Observed band size: 48 kD. |
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