Anti-ZC3H12A antibody (orb1672348) was raised against a peptide corresponding to 14 amino acids near the carboxyl terminus of human ZC3H12A. The immunogen is located within the last 50 amino acids of ZC3H12A.
Konjugation:
Unconjugated
Alternative Synonym:
ZC3H12A Antibody: Endoribonuclease ZC3H12A, Monocyte chemotactic protein-induced protein 1, MCP-induced protein 1, MCPIP-1, Zinc finger CCCH domain-containing protein 12A
ZC3H12A Antibody is supplied in PBS containing 0.02% sodium azide.
Formulierung:
Liquid
Target-Kategorie:
ZC3H12A
WB Validation in Human Lung. Loading: 15 µg of lysate per lane. Antibodies: ZC3H12A orb1672348, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 2 µg/mL. Lane 2: 4 µg/mL.
Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: ZC3H12A orb1672348, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Rat Tissues. Loading: 15 µg of lysates per lane. Antibodies: ZC3H12A orb1672348, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunofluorescence Validation of ZC3H12A in Human THP-1 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed THP-1 cells labeling ZC3H12A with orb1672348 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunohistochemistry Validation of ZC3H12A in Human Testis. Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-ZC3H12A antibody (orb1672348) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 °C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of ZC3H12A in Mouse Liver. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ZC3H12A antibody (orb1672348) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 °C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of ZC3H12A in Rat Brain. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ZC3H12A antibody (orb1672348) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 °C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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