WB: 1:1,000, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections., ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Anwendungsbeschreibung:
Application Notes: Format: Whole IgG
Detection of human LOX-1 by immunocytochemistry. Sample: FFPE section of human DLD-1 cells. Antibody: Rabbit anti-LOX-1 recombinant monoclonal antibody (orb1710818). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human LOX-1 by immunocytochemistry. Sample: FFPE section of human NCI-H226 cells. Antibody: Rabbit anti-LOX-1 recombinant monoclonal antibody (orb1710818). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human LOX-1 by immunohistochemistry. Sample: FFPE section of human gastric carcinoma. Antibody: Rabbit anti-LOX-1 recombinant monoclonal antibody (orb1710818). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human LOX-1 by immunohistochemistry. Sample: FFPE section of human placenta. Antibody: Rabbit anti-LOX-1 recombinant monoclonal antibody (orb1710818). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human LOX-1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-LOX-1 recombinant monoclonal antibody (orb1710818). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human LOX-1 by western blot. Samples: Whole cell lysate (25 µg) from DLD-1, MCF-7, and SiHa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-LOX-1 recombinant monoclonal antibody (orb1710818) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
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