Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Formulierung:
Liquid
Target-Kategorie:
Thrombomodulin
Application Verdünnung:
WB: 1:1,000, IP: 12 µl/1 mg lysate, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections., ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Anwendungsbeschreibung:
Application Notes: Format: Whole IgG
Detection of human Thrombomodulin (shaded) in THP-1 cells by flow cytometry. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (orb1710834) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.
Detection of human Thrombomodulin by immunocytochemistry. Sample: FFPE section of human SW954 cells. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (orb1710834). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human Thrombomodulin by immunocytochemistry. Sample: FFPE section of human A-431 cells. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (orb1710834). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human Thrombomodulin by immunohistochemistry. Sample: FFPE section of human placenta. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (orb1710834). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human Thrombomodulin by immunohistochemistry. Sample: FFPE section of human lymph node. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (orb1710834). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human Thrombomodulin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction, 20% of IP loaded) from A-431 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (orb1710834) used for IP at 12 µl/mg lysate.
Detection of human Thrombomodulin by western blot. Samples: Whole cell lysate (50 µg) from A-549, MDA-MB-231, A-431 (25 µg) , THP-1, and CAL-27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Thrombomodulin recombinant monoclonal antibody (orb1710834) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
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