Application : ICC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. , Application : IHC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.
Anwendungsbeschreibung:
Application Notes: Format: Whole IgG
Detection of mouse INSM1 by immunocytochemistry. Sample: FFPE section of Beta-TC-6 cells. Antibody: Rabbit anti-INSM1 recombinant monoclonal antibody (orb1733233). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human INSM1 by immunocytochemistry. Sample: FFPE section of SHP-77 cells. Antibody: Rabbit anti-INSM1 recombinant monoclonal antibody (orb1733233). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human INSM1 by immunohistochemistry. Sample: FFPE section of neuroendocrine tumor. Antibody: Rabbit anti-INSM1 recombinant monoclonal antibody (orb1733233). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human INSM1 by immunohistochemistry. Sample: FFPE section of human pancreas. Antibody: Rabbit anti-INSM1 recombinant monoclonal antibody (orb1733233). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human INSM1 by western blot. Samples: Whole cell lysate (25 µg) from HEK293T, NCI-H1155, HeLa, SHP-77, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-INSM1 recombinant monoclonal antibody (orb1733233) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of mouse INSM1 by western blot. Samples: Whole cell lysate (25 µg) from NIH 3T3, Beta-TC-6, and CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-INSM1 recombinant monoclonal antibody (orb1733233) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
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