Anti-CD47 Reference Antibody is expressed from CHO. The heavy chain type is huIgG4SP, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.
Anti-CD47 Reference Antibody (lemzoparlimab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Anti-CD47 Reference Antibody (lemzoparlimab)is more than 100% ,determined by SEC-HPLC.
Immobilized Recomvinant human CD47(c His) at1.5 µg/mL can bind Anti-CD47 Reference Antibody (lemzoparlimab),EC50=0.0163µg/mL.
PANC-1 cells were stained with Anti-CD47 Reference Antibody (lemzoparlimab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC317=0.265 ug/mL.
Anti-CD47 Reference Antibody (lemzoparlimab) FACS Blocking was evaluated using CCRF-CEM. The IC50 was approximately 0.4557 ug/mL.
The phagocytosis ratio lemzoparlimab by CCRF-CEM increased with the increase of antibody concentration, and the phagocytosis Rate (%) reached 43%.
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