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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (SOX2) Monoclonal Antibody, Unconjugated (orb1816765) at 1:1000 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse embryo), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (SOX2) Monoclonal Antibody, Unconjugated (orb1816765) at 1:1000 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (SOX2) Monoclonal Antibody, Unconjugated (orb1816765) at 1:1000 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat embryo), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (SOX2) Monoclonal Antibody, Unconjugated (orb1816765) at 1:1000 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded Human Esophagus Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with SOX2 Monoclonal Antibody, Unconjugated (orb1816765) at 1:300 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded Human Glioma, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with SOX2 Monoclonal Antibody, Unconjugated (orb1816765) at 1:300 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with SOX2 Monoclonal Antibody, Unconjugated (orb1816765) at 1:300 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |