The Goat Anti-Rabbit IgG H&L, BF488 conjugated is a BF488 conjugated polyclonal antibody suitable for FC, ICC, IF. This product reacts with Rabbit samples.
Klonalität:
Polyclonal
Konzentration:
2.0 mg/ml
Puffer:
10 mM TBS (pH=7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% glycerol.
Paraformaldehyde-fixed, paraffin embedded (human cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (GFAP) Polyclonal Antibody, Unconjugated (orb500829) at 1:100 overnight at 4C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb1817051) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (GFAP) Polyclonal Antibody, Unconjugated (orb500829) at 1:100 overnight at 4C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb1817051) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (GFAP) Polyclonal Antibody, Unconjugated (orb500829) at 1:100 overnight at 4C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb1817051) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (GFAP) Polyclonal Antibody, Unconjugated (orb500829) at 1:100 overnight at 4C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb1817051) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (GFAP) Polyclonal Antibody, Unconjugated (orb500829) at 1:100 overnight at 4C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb1817051) for 90 minutes, and DAPI for nuclei staining.
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