WB (1:1000), IHC (1:100), ICC/IF (1:150), optimal dilutions for assays should be determined by the user.
Anwendungsbeschreibung:
Application Notes: A 1:1000 dilution was sufficient for detection of Rab4 in 10 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rab4 Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Rab4 Polyclonal Antibody at 1:150 for 12 hours at 4C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Membrane. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Rab4 Antibody. (C) Composite. Heat Shocked at 42C for 30 min.
Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Rab4 protein using Rabbit Anti-Rab4 Polyclonal Antibody. Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Rab4 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
Immunohistochemistry analysis using Rabbit Anti-Rab4 Polyclonal Antibody. Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative Solution. Primary Antibody: Rabbit Anti-Rab4 Polyclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Epidermis (cell-cell border and cytoplasmic), hair follicles and muscle.
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rab4 Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Rab4 Polyclonal Antibody at 1:150 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Membrane. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Rab4 Antibody. (C) Composite. Heat Shocked at 42C for 30 min.
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rab4 Polyclonal Antibody. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-Rab4 Polyclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: String nuclear and cytoplasmic staining.
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