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HSP90 Antibody, IgG2a, Clone: [4F3.E8], Unconjugated, Mouse, Monoclonal

HSP90 Antibody, IgG2a, Clone: [4F3.E8], Unconjugated, Mouse, Monoclonal

Artikelnummer: BYT-ORB1822480

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Artikelname:	HSP90 Antibody, IgG2a, Clone: [4F3.E8], Unconjugated, Mouse, Monoclonal
Artikelnummer:	BYT-ORB1822480
Hersteller Artikelnummer:	orb1822480
Alternativnummer:	BYT-ORB1822480-200
Hersteller:	Biorbyt
Wirt:	Mouse
Kategorie:	Antikörper
Applikation:	ELISA, ICC, IF, IHC, IP, WB
Spezies Reaktivität:	Human, Mouse, Plant, Rat
Immunogen:	Recombinant Human HSP90 purified from E.coli
Konjugation:	Unconjugated
Alternative Synonym:	HSP90, HSP90AB1, HSP90-beta, HSPCB, HSPC2, Heat shock protein HSP 90-beta, Heat shock 84 kDa protein, HSP84, HSP90B

Mouse Anti-Human HSP90 (total) Monoclonal IgG2a

Klonalität:	Monoclonal
Konzentration:	1 mg/ml
Klon-Bezeichnung:	[4F3.E8]
Molekulargewicht:	90kDa
Isotyp:	IgG2a
NCBI:	001017963
UniProt:	P07900
Puffer:	PBS pH 7.2, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
Target-Kategorie:	HSP90

Application Verdünnung:	WB (1:2000), IHC (1:100), ICC/IF (1:100), optimal dilutions for assays should be determined by the user.
Anwendungsbeschreibung:	Application Notes: 0.5 µg/ml of SMC-149 was sufficient for detection of HSP90alpha in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody

	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
	Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.
	Western Blot analysis of Rat tissue lysate showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8. Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
	Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8. Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
	Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8. Tissue: muscle tissue. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

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