BMP2 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB182463
Artikelname: BMP2 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB182463
Hersteller Artikelnummer: orb182463
Alternativnummer: BYT-ORB182463-50,BYT-ORB182463-100,BYT-ORB182463-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human BMP2 (341-396/396aa)
Konjugation: Unconjugated
Alternative Synonym: BDA2, BMP2A, SSFSC, SSFSC1, BMP2_HUMAN, BMP2, BMP-2, Bone morphogenetic protein 2A (BMP-2A), BMP2_MOUSE, BMP2_RAT,
BMP2 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 13/44 kDa
Pubmed: 29174869
UniProt: P12643
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: BMP2
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical staining of rat stomach tissue using BMP2 antibody.
Western blot analysis of mouse Eye lysates (Lane 1) using BMP2 antibody.
Sample: A431 (Human) Cell Lysate at 30 ug, Hela (Human) Cell Lysate at 30 ug, Primary: Anti-BMP2 (orb182463) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 13/44 kD, Observed band size: 64 kD.
Sample: Stomach (Mouse) Lysate at 40 ug, Primary: Anti-BMP2 (orb182463) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 13/44 kD, Observed band size: 59 kD.
Tissue/Cell: rat stomach tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-BMP2 Polyclonal Antibody, Unconjugated (orb182463) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.



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