Immunohistochemical staining of rat testis tissue using C19orf51 antibody.
Immunohistochemical staining of rat brain tissue using C19orf51 antibody.
Sample: Cerebellum (Mouse) Lysate at 40 ug, Primary: Anti-C19orf51 (orb182488) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 59 kD, Observed band size: 64 kD.
Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-C19orf51 Polyclonal Antibody, Unconjugated (orb182488) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-C19orf51 Polyclonal Antibody, Unconjugated (orb182488) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: rat testis tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-C19orf51 Polyclonal Antibody, Unconjugated (orb182488) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
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