CRB2 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB182732
Artikelname: CRB2 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB182732
Hersteller Artikelnummer: orb182732
Alternativnummer: BYT-ORB182732-50,BYT-ORB182732-100,BYT-ORB182732-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human CRB2 (701-800/1285aa)
Konjugation: Unconjugated
Alternative Synonym: FSGS9, VMCKD, 5930402A21, RGD1309368, CRUM2_HUMAN, CRB2, Crumbs-like protein 2, CRUM2_MOUSE, crumbs2,
CRB2 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 100, 123, 131 kDa
UniProt: Q5IJ48
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: CRB2
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CRB2) Polyclonal Antibody, Unconjugated (orb182732) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-CRB2 (orb182732) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 100, 123, 131 kD, Observed band size: 100, 131 kD.
Sample: Eye (Rat) Lysate at 40 ug, Primary: Anti-CRB2 (orb182732) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 100/123/131 kD, Observed band size: 135 kD.
Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-CRB2 Polyclonal Antibody, Unconjugated (orb182732) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: rat kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-CRB2 Polyclonal Antibody, Unconjugated (orb182732) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.



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