Phospho-Cyclin C (Ser275) Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB182829
- Bilder (7)
| Artikelname: | Phospho-Cyclin C (Ser275) Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB182829 |
| Hersteller Artikelnummer: | orb182829 |
| Alternativnummer: | BYT-ORB182829-50,BYT-ORB182829-100,BYT-ORB182829-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated Synthesised phosphopeptide derived from human Cyclin C around the phosphorylation site of Ser275 NG(p-S)QN |
| Konjugation: | Unconjugated |
| Alternative Synonym: | CCNC | Cyclin-C (p-S275), p-Cyclin-C, phospho-Cyclin-C, CycC, SRB11, hSRB11, CG1C, CCNC_HUMAN, CCNC, SRB11 homolog (hSRB11), CCNC_MOUSE, CCNC_RAT, |
| Phospho-Cyclin C (Ser275) Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 33 kDa |
| UniProt: | P24863 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | CCNC |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Anwendungsbeschreibung: | Modification: Phosphorylated |
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Immunohistochemical staining of rat pancreas tissue using Cyclin C (phospho-Ser275) antibody. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Cyclin C (Ser275)) Polyclonal Antibody, Unconjugated (orb182829) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Cyclin C (Ser275)) Polyclonal Antibody, Unconjugated (orb182829) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Cyclin C (Ser275)) Polyclonal Antibody, Unconjugated (orb182829) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Panc-1 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-Cyclin C (Ser275) (orb182829) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 33 kD, Observed band size: 35 kD. |
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Sample: Pancreas (Mouse) Lysate at 40 ug, Primary: Anti-phospho-Cyclin C (Ser275) (orb182829) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 33 kD, Observed band size: 22 kD. |
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Tissue/Cell: rat pancreas tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-phospho-Cyclin C (Ser275) Polyclonal Antibody, Unconjugated (orb182829) 1:400, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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