COX4I1 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB182884
Artikelname: COX4I1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB182884
Hersteller Artikelnummer: orb182884
Alternativnummer: BYT-ORB182884-50,BYT-ORB182884-100,BYT-ORB182884-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human COX4I1 (51-169/169aa)
Konjugation: Unconjugated
Alternative Synonym: COX IV-1, COX4, COX4-1, COXIV, COXIV-1, MC4DN16, COX, Cox4a, IV-1, COX41_HUMAN, COX4I1, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit IV isoform 1 (COX IV-1), COX41_MOUSE, COX41_RAT,
COX4I1 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 17 kDa
UniProt: P13073
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: COX4I1
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Flow cytometric analysis of Hela cells using COX4 antibody.
Western blot analysis of extracts from kidney using COX4 antibody.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (COX4) Polyclonal Antibody, Unconjugated (orb182884) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Sample: Lane 1: Mouse Kidney tissue lysates, Lane 2: Mouse Heart tissue lysates, Lane 3: Mouse Muscle tissue lysates, Lane 4: Rat Kidney tissue lysates, Lane 5: Rat Heart tissue lysates, Lane 6: Rat Muscle tissue lysates, Lane 7: Human HeLa cell lysates, Lane 8: Human Jurkat cell lysates, Lane 9: Human THP-1 cell lysates, Lane 10: Human Raji cell lysates, Lane 11: Human HepG2 cell lysates, Lane 12: Human MCF-7 cell lysates, Primary: Anti-COX4I1 (orb182884) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 17 kDa, Observed band size: 16 kDa.
Tissue/Cell: Rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-COX4 Polyclonal Antibody, Unconjugated (orb182884) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.



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