Collagen II Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB182930
- Bilder (7)
| Artikelname: | Collagen II Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB182930 |
| Hersteller Artikelnummer: | orb182930 |
| Alternativnummer: | BYT-ORB182930-50,BYT-ORB182930-100,BYT-ORB182930-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse |
| Immunogen: | KLH conjugated synthetic peptide derived from human Collagen II (231-330/1487aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | ACG2, ANFH, ANFH1, AOM, COL11A3, EDMMD, LCPD, OSCDP, PLSDT, SEDC, SEDSTN, SEMDSTWK, SMDALG, STL1, VPED, Col2, Col2a, Col2a-1, Del1, Dmm, Lpk, M100413, Rgsc413, Rgsc856, CG2A1A, COLLII, CO2A1_HUMAN, COL2A1, Alpha-1 type II collagen, CO2A1_MOUSE, CO2A1_RAT, |
| Collagen II Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 117 kDa |
| UniProt: | P02458 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | COL2A1 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Immunohistochemical staining of Auricle tissue using Collagen II antibody. |
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Immunohistochemical staining of Mouse embryo tissue using Collagen II antibody. |
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Paraformaldehyde-fixed, paraffin embedded (mouse ear), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Collagen II) Polyclonal Antibody, Unconjugated (orb182930) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Sample: 293T (Human) Cell Lysate at 30 ug, A549 (Human) Cell Lysate at 30 ug, LOVO (Human) Cell Lysate at 40 ug, MCF-7 (Human) Cell Lysate at 30 ug, HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-Collagen II (orb182930) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 117 kD, Observed band size: 117 kD. |
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Sample: bone (Mouse) Lysate at 40 ug, Primary: Anti-Collagen II (orb182930) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 117 kD, Observed band size: 134 kD. |
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Sample: MG63 (Human) Cell Lysate at 30 ug, Primary: Anti-Collagen II (orb182930) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 117 kD, Observed band size: 117 kD. |
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Tissue/Cell: Mouse embryo tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Collagen II Polyclonal Antibody, Unconjugated (orb182930) 1:100, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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