CLEC16A Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB182958
- Bilder (7)
| Artikelname: | CLEC16A Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB182958 |
| Hersteller Artikelnummer: | orb182958 |
| Alternativnummer: | BYT-ORB182958-100,BYT-ORB182958-200,BYT-ORB182958-50 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human |
| Immunogen: | KLH conjugated synthetic peptide derived from human CLEC16A |
| Konjugation: | Unconjugated |
| Alternative Synonym: | Gop-1, KIAA0350, 4932416N17Rik, curt, mKIAA0350, CL16A_HUMAN, CLEC16A, C-type lectin domain family 16 member A, CL16A_MOUSE, |
| CLEC16A Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 118 kDa |
| UniProt: | Q2KHT3 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | CLEC16A |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Immunohistochemical staining of human colon carcinoma tissue using CLEC16A antibody. |
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Western blot analysis of human K562 Cell lysates (Lane 1) using CLEC16A antibody. |
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Paraformaldehyde-fixed, paraffin embedded (human lung cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CLEC16A) Polyclonal Antibody, Unconjugated (orb182958) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Sample: K562 (Human) Cell Lysate at 30 ug, Siha (Human) Cell Lysate at 30 ug, U-2OS (Human) Cell Lysate at 30 ug, U87MG (Human) Cell Lysate at 30 ug, Primary: Anti-CLEC16A (orb182958) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 118 kD, Observed band size: 118 kD. |
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Sample: k562 (human) cell Lysate at 40 ug, Primary: Anti-CLEC16A (orb182958) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 118 kD, Observed band size: 118 kD. |
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Tissue/Cell: human colon carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-CLEC16A Polyclonal Antibody, Unconjugated (orb182958) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-CLEC16A Polyclonal Antibody, Unconjugated (orb182958) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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