DHX36 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB183101
Artikelname: DHX36 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB183101
Hersteller Artikelnummer: orb183101
Alternativnummer: BYT-ORB183101-50,BYT-ORB183101-100,BYT-ORB183101-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human DHX36 (151-250/1008aa)
Konjugation: Unconjugated
Alternative Synonym: DDX36, G4R1, MLEL1, RHAU, 2810407E23Rik, mKIAA1488, DHX36_HUMAN, DHX36, DEAD/H box polypeptide 36, DEAH-box protein 36, G4-resolvase-1 (G4R1), MLE-like protein 1, RNA helicase associated with AU-rich element protein, 3.6.4.12, 3.6.4.13, KIAA1488, DHX36_MOUSE, DEAH box protein 36, RNA helicase associated with AU-rich element ARE,
DHX36 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 115 kDa
UniProt: Q9H2U1
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: DHX36
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (DHX36) Polyclonal Antibody, Unconjugated (orb183101) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (DHX36) Polyclonal Antibody, Unconjugated (orb183101) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (DHX36) Polyclonal Antibody, Unconjugated (orb183101) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-DHX36 (orb183101) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 115 kD, Observed band size: 115 kD.
Sample: Testis (Mouse) Lysate at 40 ug, Primary: Anti-DHX36 (orb183101) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 115 kD, Observed band size: 115 kD.



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