DPAGT1 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB183188
Artikelname: DPAGT1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB183188
Hersteller Artikelnummer: orb183188
Alternativnummer: BYT-ORB183188-50,BYT-ORB183188-100,BYT-ORB183188-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human DPAGT1 (301-408/408aa)
Konjugation: Unconjugated
Alternative Synonym: ALG7, CDG-Ij, CDG1J, CMS13, CMSTA2, D11S366, DGPT, DPAGT, DPAGT2, G1PT, GPT, UAGT, UGAT, Gnpta, GPT_HUMAN, DPAGT1, GlcNAc-1-P transferase (G1PT | GPT), N-acetylglucosamine-1-phosphate transferase, 2.7.8.15, GPT_MOUSE,
DPAGT1 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 46 kDa
UniProt: Q9H3H5
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: DPAGT1
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical staining of rat brain tissue using DPAGT1 antibody.
Western blot analysis of mouse lung tissue using DPAGT1 antibody.
Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (DPAGT1) Polyclonal Antibody, Unconjugated (orb183188) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Protein: lung (mouse) lysate at 40 ug, Primary: rabbit Anti-DPAGT1 (orb183188) at 1:300, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000, Predicted band size: 46 kD, Observed band size: 46 kD.
Tissue/Cell: Rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-DPAGT1 Polyclonal Antibody, Unconjugated (orb183188) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.



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