Kv1.8 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB184609
Artikelname: Kv1.8 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB184609
Hersteller Artikelnummer: orb184609
Alternativnummer: BYT-ORB184609-50,BYT-ORB184609-100,BYT-ORB184609-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human Kv1.8 (301-400/511aa)
Konjugation: Unconjugated
Alternative Synonym: Kcn1, Kv1.8, Gm1962, Kcna8, KCA10_HUMAN, KCNA10, Voltage-gated potassium channel subunit Kv1.8,
Kv1.8 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 58 kDa
UniProt: Q16322
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: KCNA10
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical staining of human lung carcinoma tissue using Kv1.8 antibody.
Immunohistochemical staining of rat kidney tissue using Kv1.8 antibody.
Protein: stomach (mouse) lysates at 40 ug, kidney (mouse) lysates at 40 ug, Primary: Anti-Kv1.8 (orb184609) at 1:300, Secondary: HRP conjugated Goat-Anti-Rabbit IgG (orb572747) at 1:5000, ECL excitated the fluorescence, Predicted band size: 58 kD, Observed band size: 58 kD.
Sample: Muscle (Mouse) Lysate at 40 ug, Primary: Anti-Kv1.8 (orb184609) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 58 kD, Observed band size: 58 kD.
Sample: Stomach (Mouse) Lysate at 40 ug, Primary: Anti-Kv1.8 (orb184609) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 58 kD, Observed band size: 58 kD.
Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Kv1.8 Polyclonal Antibody, Unconjugated (orb184609) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: rat kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Kv1.8 Polyclonal Antibody, Unconjugated (orb184609) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.



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