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Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (LULL1) Polyclonal Antibody, Unconjugated (orb184866) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (LULL1) Polyclonal Antibody, Unconjugated (orb184866) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: K562 (Human) Cell Lysate at 30 ug, Lane 2: SH-SY5Y (Human) Cell Lysate at 30 ug, Lane 3: Hela (Human) Cell Lysate at 30 ug, Lane 4: HUVEC (Human) Cell Lysate at 30 ug, Lane 5: U2os (Human) Cell Lysate at 30 ug, Lane 6: HepG2 (Human) Cell Lysate at 30 ug, Lane 7: Liver (Mouse) Lysate at 40 ug, Lane 8: Liver (Rat) Lysate at 40 ug, Lane 9: Kidney (Mouse) Lysate at 40 ug, Lane 10: Cerebrum (Mouse) Lysate at 40 ug, Lane 11: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-LULL1/TOR1AIP2 (orb184866) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51 kD, Observed band size: 51kD. |
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Sample: Placenta (Mouse) Lysate at 40 ug, Large intestine (Mouse) Lysate at 40 ug, Primary: Anti-LULL1/TOR1AIP2 (orb184866) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51kD, Observed band size: 56kD. |
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Sample: Small intestine (Mouse) Lysate at 40 ug, Primary: Anti-LULL1/TOR1AIP2 (orb184866) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51kD, Observed band size: 56kD. |
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IHC-P analysis of rat kidney using LULL1 Polyclonal Antibody at 1:200 dilution. |
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IHC-P analysis of mouse kidney using LULL1 Polyclonal Antibody at 1:200 dilution. |