Nanog Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB185639
- Bilder (7)
| Artikelname: | Nanog Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB185639 |
| Hersteller Artikelnummer: | orb185639 |
| Alternativnummer: | BYT-ORB185639-50,BYT-ORB185639-100,BYT-ORB185639-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from mouse Nanog (101-200/305aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | 2410002E02Rik, ENK, Stm1, ecat4, NANOG_HUMAN, NANOG, Homeobox transcription factor Nanog (hNanog), |
| Nanog Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 34 kDa |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | NANOG |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (Mouse ovarian), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Nanog) Polyclonal Antibody, Unconjugated (orb185639) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse ovary), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Nanog) Polyclonal Antibody, Unconjugated (orb185639) at 1:400 overnight at 4C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb868589) for 90 minutes, and DAPI for nuclei staining. |
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Sample: A431 (Human) Cell Lysate at 30 ug, Primary: Anti-Nanog (orb185639) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kD, Observed band size: 34 kD. |
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Sample: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Primary: Anti-Nanog (orb185639) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kD, Observed band size: 34 kD. |
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Tissue/Cell: mouse embryo tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Nanog Polyclonal Antibody, Unconjugated (orb185639) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: rat testis tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Nanog Polyclonal Antibody, Unconjugated (orb185639) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: rat testis tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Nanog Polyclonal Antibody, Unconjugated (orb185639) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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