SC35 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB186058
Artikelname: SC35 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB186058
Hersteller Artikelnummer: orb186058
Alternativnummer: BYT-ORB186058-50,BYT-ORB186058-100,BYT-ORB186058-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse
Immunogen: KLH conjugated synthetic peptide derived from human SC35 (131-221/221aa)
Konjugation: Unconjugated
Alternative Synonym: SFRS2, PR264, SC-35, SC35, SFRS2A, SRp30b, D11Wsu175e, MRF-1, Sfrs10, SRSF2_CHICK, SRSF2, Protein PR264, Splicing component, 35 kDa, Splicing factor SC35 (SC-35), Splicing factor, arginine/serine-rich 2, SRSF2_BOVIN, SRSF2_HUMAN, SRSF2_MOUSE, Putative myelin regulatory factor 1 (MRF-1), SRSF2_RAT,
SC35 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 25 kDa
UniProt: Q01130
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: SRSF2
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SC35) Polyclonal Antibody, Unconjugated (orb186058) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SC35) Polyclonal Antibody, Unconjugated (orb186058) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SC35) Polyclonal Antibody, Unconjugated (orb186058) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Primary: Anti-SC35 (orb186058) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 25 kD, Observed band size: 25 kD.
Western blot analysis of NIH/3T3(Mouse) Cell Lysate using SC35 antibody.
Immunohistochemical staining of Mouse brain using SC35 antibody.
Immunohistochemical staining of Mouse testis using SC35 antibody.



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