anti AKT3 antibody, anti STK-2 antibody, anti RAC-gamma antibody, anti RAC-PK-gamma antibody, anti DKFZP434N0250 antibody, anti protein kinase B gamma antibody, anti serine threonine protein kinase, Akt-3 antibody, anti RAC-gamma serine/threonine protein kinase antibody
Goat polyclonal antibody to AKT3
Klonalität:
Polyclonal
Molekulargewicht:
55.8, 54.0
Puffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Sequenz:
CSPTSQIDNIGEEEM
Target-Kategorie:
AKT3
Application Verdünnung:
ELISA: 1:16000, WB: 1-3 µg/ml, IHC-P: 5 µg/ml
Anwendungsbeschreibung:
Application Notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: A 50-55kDa band observed in Human Hepatoblastoma HepG2 lysates (calculated MW of 55.8kDa according to NP_005456 and 54.0kDa according to NP_859029). Recommended concentration: 1-3 µg/ml
1 µg/ml staining of HepG2 (A) and HeLa (B) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
1 µg/ml staining of Human Thyroid (A), (0.5 ug/ml) Mouse Brain (B) and (1 µg/ml) Rat Brain (C) lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
5 µg/ml staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
5 µg/ml staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunofluorescence analysis of paraformaldehyde fixed THP-1 immobilized on Shi-fix(TM) plus cover-slips. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor 488 secondary antibody (1:2000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Anti-Goat IgG followed by Alexa Fluor 488 secondary antibody.
Immunofluorescence analysis of paraformaldehyde fixed A431. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor 488 secondary antibody (1:2000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Anti-Goat IgG followed by Alexa Fluor 488 secondary antibody.
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