Rat DNA Methyltransferase 3B (DNMT3B) ELISA Kit

Artikelnummer: BYT-ORB1947935
Artikelname: Rat DNA Methyltransferase 3B (DNMT3B) ELISA Kit
Artikelnummer: BYT-ORB1947935
Hersteller Artikelnummer: orb1947935
Alternativnummer: BYT-ORB1947935-48,BYT-ORB1947935-96
Hersteller: Biorbyt
Kategorie: Kits/Assays
Spezies Reaktivität: Rat
Alternative Synonym: CF, M.HsaIIIB, DNA MTase HsaIIIB, DNA cytosine-5 methyltransferase 3 beta, DNA methyltransferase HsaIIIB
Rat DNA Methyltransferase 3B (DNMT3B) ELISA Kit
Detektionsbereich: 0.16-10ng/mL
Sensitivitaet: 0.1 ng/mL
Proben: serum, plasma, Tissue homogenate and Other biological samples
Target-Kategorie: DNMT3B
Anwendungsbeschreibung: Application Notes: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat DNMT3B. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat DNMT3B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat DNMT3B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of Rat DNMT3B. You can calculate the concentration of Rat DNMT3B in the samples by comparing the OD of the samples to the standard curve
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