Mouse Phospho-Inhibitory Subunit of NF Kappa B Alpha (pIkappaBalpha) ELISA Kit
Artikelnummer:
BYT-ORB1948720
Hersteller Artikelnummer:
orb1948720
Alternativnummer:
BYT-ORB1948720-48,BYT-ORB1948720-96
Hersteller:
Biorbyt
Kategorie:
Kits/Assays
Spezies Reaktivität:
Mouse
Alternative Synonym:
pIkappaBalpha
Mouse Phospho-Inhibitory Subunit of NF Kappa B Alpha (pIkappaBalpha) ELISA Kit
Detektionsbereich:
0.16-10ng/mL
Sensitivitaet:
0.1 ng/mL
Proben:
Tissue homogenate and Cell lysate
Target-Kategorie:
pIkappaBalpha
Anwendungsbeschreibung:
Modification: Phosphorylated. Application Notes: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse pIkappaBalpha. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse pIkappaBalpha and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse pIkappaBalpha, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of Mouse pIkappaBalpha. You can calculate the concentration of Mouse pIkappaBalpha in the samples by comparing the OD of the samples to the standard curve
orb1948720
orb1948720
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