anti GAD1 antibody, anti glutamate decarboxylase 1 (brain, 67kDa) antibody, anti FLJ45882 antibody, anti GAD antibody, anti SCP antibody, anti OTTHUMP00000041055 antibody, anti glutamate decarboxylase 1 antibody, anti glutamate decarboxylase 1 (brain, 67kD) antibody, anti GAD67 antibody, anti GAD25 antibody
Goat polyclonal antibody to GAD1
Klonalität:
Polyclonal
Molekulargewicht:
66.9
Puffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Sequenz:
PDSPQRREKLHK
Target-Kategorie:
GAD1 (isoform GAD67)
Application Verdünnung:
ELISA: 1:32000, WB: 0.5-1.5 µg/ml
Anwendungsbeschreibung:
Application Notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx. 70kDa band observed in Human Brain (Cerebellum) and in Mouse and Rat Brain lysates (calculated MW of 66.9kDa according to Human NP_000808.2 and 66.6kDa according to Mouse NP_032103.2). Recommended concentration: 0.3-1 µg/ml
orb20081 (1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb20081 (1µg/ml) staining of Human Cerebellum (A) and Mouse Brain (B) and (0.3ug/ml) Rat Brain (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
orb20081 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
orb20081 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Western blot analysis of Human Brain lysate using GAD1 antibody
Immunohistochemical staining of Human Cerebellum using GAD1 antibody
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