Application Notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 51.4 kDa. The protein migrates as 64-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation
SDS-PAGE analysis of Human IFN-alpha/beta R2 protein
Loaded Human IFN-alpha 1, His Tag (Cat. No. orb735041) on HIS1K Biosensor, can bind Human IFNAR2, Fc Tag (Cat. No. orb257561) with an affinity constant of 19.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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