Application Notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 61.0 kDa. The protein migrates as 80-100 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation
SDS-PAGE analysis of Human IL-1RL1 protein
Loaded Human IL-1RL1, Fc Tag (Cat. No. orb257583) on Protein A Biosensor, can bind with Biotinylated Human IL-33, His, Avitag an affinity constant of 1.81 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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