Application Notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 38.9 kDa. The protein migrates as 50-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation
SDS-PAGE analysis of Human IL-1 RII protein
Immobilized Human IL-1 beta, Fc Tag (Cat. No. orb752327) at 5 µg/mL (100 µL/well) can bind Human IL-1 RII, His Tag (Cat. No. orb257591) with a linear range of 1-20 ng/mL (QC tested).
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