0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung:
Liquid
Target-Kategorie:
ENG
Application Verdünnung:
IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical staining of human placenta tissue using CD105 antibody.
Immunohistochemical staining of human gastric carcinoma tissue using CD105 antibody.
Blank control: Hela. Primary Antibody (green line): Rabbit Anti-CD105 antibody (orb312175), dilution: 2 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD105 Polyclonal Antibody, Unconjugated (orb312175) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD105 Polyclonal Antibody, Unconjugated (orb312175) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD105 Polyclonal Antibody, Unconjugated (orb312175) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
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