CHRNA7 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB312180
Artikelname: CHRNA7 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB312180
Hersteller Artikelnummer: orb312180
Alternativnummer: BYT-ORB312180-50,BYT-ORB312180-100,BYT-ORB312180-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human CHRNA7 (441-502/502aa)
Konjugation: Unconjugated
Alternative Synonym: CHRNA7-2, NACHRA7, nAChR7, Acra7, alpha7, nAchR, BTX, NARAD, ACHA7_CHICK, CHRNA7, Nicotinic acetylcholine receptor subunit alpha-7, ACHA7_BOVIN, ACHA7_HUMAN, Q8IUZ4_HUMAN, ACHA7_MOUSE, ACHA7_RAT,
CHRNA7 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 55 kDa
Pubmed: 41558592
UniProt: P36544
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: CHRNA7
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical staining of Mouse brain tissue using CHRNA7 antibody.
Immunohistochemical staining of human kidney tissue using CHRNA7 antibody.
Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CHRNA7) Polyclonal Antibody, Unconjugated (orb312180) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Cerebrum (Rat) Lysate at 40 ug, Lane 2: Cerebrum (Mouse) Lysate at 40 ug, Lane 3: Adrenal glands (Rat) Lysate at 40 ug, Lane 4: Adrenal glands (Mouse) Lysate at 40 ug, Lane 5: Placenta (Mouse) Lysate at 40 ug, Lane 6: Lung (Mouse) Lysate at 40 ug, Lane 7: Testis (Rat) Lysate at 40 ug, Lane 8: HepG2 (Human) Cell Lysate at 30 ug, Lane 9: SH-SY5Y (Human) Cell Lysate at 30 ug, Primary: Anti-CHRNA7 (orb312180) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 5650 kD, Observed band size: 48 kD.
Sample: Lane 1: Human SH-SY5Y cell Lysates, Lane 2: Human U-87 MG cell Lysates, Lane 3: Human U251 cell Lysates, Primary: Anti-CHRNA7 (orb312180) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55kDa, Observed band size: 55kDa.
Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-CHRNA7 Polyclonal Antibody, Unconjugated (orb312180) 1:200, overnight at 4C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.



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