MCT1/Malignant T cell amplified sequence 1 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB312305
Artikelname: MCT1/Malignant T cell amplified sequence 1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB312305
Hersteller Artikelnummer: orb312305
Alternativnummer: BYT-ORB312305-50,BYT-ORB312305-100,BYT-ORB312305-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: FC, IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human MCT1 (34-59/500aa)
Konjugation: Unconjugated
Alternative Synonym: HHF7, MCT, MCT1, MCT1D, MOT1_HUMAN, SLC16A1, MCT 1, Solute carrier family 16 member 1, MOT1_MOUSE,
MCT1/Malignant T cell amplified sequence 1 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 55 kDa
UniProt: P53985
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: SLC16A1
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test
Flow cytometric analysis of Mouse spleen cell using MCT1 antibody.
Immunohistochemical staining of rat brain tissue using MCT1 antibody.
Sample: Brain (Rat) lysate at 45 ug, Colon carcinoma (Human) lysate at 45 ug, Primary: Anti-MCT1 (orb312305) at 1:200, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:3000, Predicted band size: 55kD, Observed band size: 55kD.
Sample: Lane 1: Liver (Mouse) Lysate at 40 ug, Lane 2: Stomach (Mouse) Lysate at 40 ug, Lane 3: Heart (Mouse) Lysate at 40 ug, Lane 4: Liver (Rat) Lysate at 40 ug, Lane 5: Stomach (Rat) Lysate at 40 ug, Lane 6: Heart (Rat) Lysate at 40 ug, Primary: Anti-MCT1 (orb312305) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 48 kD.
Tissue/Cell: human colon carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-MCT1 Polyclonal Antibody, Unconjugated (orb312305) 1:200, overnight at 4C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37C.
Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-MCT1 Polyclonal Antibody, Unconjugated (orb312305) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.



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