PSME1 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB312512
Artikelname: PSME1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB312512
Hersteller Artikelnummer: orb312512
Alternativnummer: BYT-ORB312512-50,BYT-ORB312512-100,BYT-ORB312512-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human PSME1 (181-249/249aa)
Konjugation: Unconjugated
Alternative Synonym: HEL-S-129m, IFI5111, PA28A, PA28alpha, REGalpha, PSME1_BOVIN, PSME1, Proteasome activator 28 subunit alpha (PA28a | PA28alpha), PSME1_HUMAN, 11S regulator complex subunit alpha (REG-alpha), Activator of multicatalytic protease subunit 1, Interferon gamma up-regulated I-5111 protein (IGUP I-5111), PSME1_MOUSE, PSME1_PIG, PSME1_RAT,
PSME1 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 29 kDa
UniProt: Q06323
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: PSME1
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (rat lung tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PSME1) Polyclonal Antibody, Unconjugated (orb312512) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat spleen tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PSME1) Polyclonal Antibody, Unconjugated (orb312512) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Protein: spleen (mouse) lysate at 40 ug, Primary: rabbit Anti-PSME1 (orb312512) at 1:300, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 29 kD, Observed band size: 29 kD.
Sample: Spleen (mouse) Lysate at 40 ug, Primary: Anti-PSME1 (orb312512) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 29kD, Observed band size: 29kD.
Immunohistochemical staining of Rat lung tissue using PSME1 antibody.
Western blot analysis of mouse spleen cell lysates (Lane 1) using PSME1 antibody.



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