KLH conjugated synthetic peptide derived from human PSMG1 (21-100/288aa)
Konjugation:
Unconjugated
Alternative Synonym:
C21LRP, DSCR2, LRPC21, PAC-1, PAC1, Pba1, PSMG1_HUMAN, PSMG1, Chromosome 21 leucine-rich protein (C21-LRP), Down syndrome critical region protein 2, Proteasome chaperone homolog 1 (Pba1), PSMG1_MOUSE, Down syndrome critical region protein 2 homolog,
Protein: brain (mouse) lysate at 40 ug, Primary: rabbit Anti-PSME1 at 1:300, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 29 kD, Observed band size: 29 kD.
Tissue/Cell: human brain glioma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-PSMG1 Polyclonal Antibody, Unconjugated (orb312514) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: human breast carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-PSMG1 Polyclonal Antibody, Unconjugated (orb312514) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: human cervix carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-PSMG1 Polyclonal Antibody, Unconjugated (orb312514) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemical staining of human cervix carcinoma tissue using PSMG1 antibody.
Western blot analysis of mouse Brain lysates (Lane 1) using PSMG1 antibody.
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