Phospho-Parkin (Ser65) Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB312554
Artikelname: Phospho-Parkin (Ser65) Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB312554
Hersteller Artikelnummer: orb312554
Alternativnummer: BYT-ORB312554-50,BYT-ORB312554-100,BYT-ORB312554-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse
Immunogen: KLH conjugated synthesised phosphopeptide derived from human Parkin around the phosphorylation site of Ser65 QQ(p-S)IV
Konjugation: Unconjugated
Alternative Synonym: PRKN | Parkin (p-S65), p-Parkin, phospho-Parkin, AR-JP, LPRS2, PARK2, PDJ, PRKN_HUMAN, PRKN, Parkin, Parkin RBR E3 ubiquitin-protein ligase, Parkinson juvenile disease protein 2 (Parkinson disease protein 2), 2.3.2.31,
Phospho-Parkin (Ser65) Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 52 kDa
Pubmed: 35030984
UniProt: O60260
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: PRKN
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Anwendungsbeschreibung: Modification: Phosphorylated
Western blot analysis of A673 Cell Lysate using Parkin antibody
Immunohistochemical staining of human brain glioma using Parkin antibody
Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Parkin (Ser65)) Polyclonal Antibody, Unconjugated (orb312554) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: A673 (Human) cell Lysate at 40 ug, Primary: Anti-p-Parkin (Ser65) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kD, Observed band size: 52 kD.
Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug Lane 2: Lung (Mouse) Lysate at 40 ug Lane 3: SH-SY5Y (Human) Cell Lysate at 30 ug Lane 4: 293T (Human) Cell Lysate at 30 ug Lane 5: MCF-7 (Human) Cell Lysate at 30 ug Lane 6: Jurkat (Human) Cell Lysate at 30 ug Primary: Anti-phospho-Parkin (Ser65) (orb312554) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 50 kD.
Sample: Thymus (Mouse) Lysate at 40 ug Liver (Mouse) Lysate at 40 ug Lung (Mouse) Lysate at 40 ug Skeletal muscle (Mouse) Lysate at 40 ug Primary: Anti-phospho-Parkin (Ser65) (orb312554) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD.



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