Phospho-PDCD4 (Ser67) Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB312714
- Bilder (6)
| Artikelname: | Phospho-PDCD4 (Ser67) Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB312714 |
| Hersteller Artikelnummer: | orb312714 |
| Alternativnummer: | BYT-ORB312714-50,BYT-ORB312714-100,BYT-ORB312714-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | FC, ICC, IF, IHC-Fr, IHC-P |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthesised phosphopeptide derived from human PDCD4 around the phosphorylation site of Ser67 KN(p-S)SR |
| Konjugation: | Unconjugated |
| Alternative Synonym: | PDCD4 (p-S67), p-PDCD4, phospho-PDCD4, H731, D19Ucla1, Ma3, Tis, Dug, PDCD4_HUMAN, PDCD4, Neoplastic transformation inhibitor protein, Nuclear antigen H731-like, Protein 197/15a, PDCD4_MOUSE, Protein MA-3, Topoisomerase-inhibitor suppressed protein, PDCD4_RAT, Death up-regulated gene protein, |
| Phospho-PDCD4 (Ser67) Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 51 kDa |
| UniProt: | Q53EL6 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | PDCD4 |
| Application Verdünnung: | IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test |
| Anwendungsbeschreibung: | Modification: Phosphorylated |
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Blank control (black line): HepG2. Primary Antibody (green line): Rabbit Anti-phospho-PDCD4 (Ser67) antibody (orb312714), dilution: 2 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
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HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (phospho-PDCD4 (Ser67)) polyclonal Antibody, Unconjugated (orb312714) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Paraformaldehyde-fixed, paraffin embedded (mouse lymphoid), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (p-PDCD4 (Ser67)) Polyclonal Antibody, Unconjugated (orb312714) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (phospho-PDCD4 (Ser67)) Polyclonal Antibody, Unconjugated (orb312714) at 1:100 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat thyroid gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (phospho-PDCD4 (Ser67)) Polyclonal Antibody, Unconjugated (orb312714) at 1:100 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat thyroid gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (phospho-PDCD4 (Ser67)) Polyclonal Antibody, Unconjugated (orb312714) at 1:100 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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