Iduronate 2 sulfatase Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB317636
Artikelname: Iduronate 2 sulfatase Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB317636
Hersteller Artikelnummer: orb317636
Alternativnummer: BYT-ORB317636-50,BYT-ORB317636-100,BYT-ORB317636-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human
Immunogen: KLH conjugated synthetic peptide derived from human Iduronate 2 sulfatase (101-200/550aa)
Konjugation: Unconjugated
Alternative Synonym: ID2S, MPS2, SIDS, IDS_HUMAN, IDS, Alpha-L-iduronate sulfate sulfatase (Idursulfase), 3.1.6.13,
Iduronate 2 sulfatase Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 47 kDa
UniProt: P22304
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: IDS
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Iduronate 2 sulfatase) Polyclonal Antibody, Unconjugated (orb317636) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: 293T Cell (Human) Lysate at 30 ug, Primary: Anti-Iduronate 2 sulfatase (orb317636) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47kD, Observed band size: 62kD.
Sample: Hela Cell (Human) Lysate at 30 ug, Primary: Anti-Iduronate 2 sulfatase (orb317636) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47kD, Observed band size: 62kD.
IHC-P analysis of Human kidney using Iduronate 2 sulfatase Polyclonal Antibody at 1:200 dilution.
Western blot:293T Cell,(Human),Primary:Anti-Iduronate 2 sulfatase at 1:300 dilution.



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