QPRT/QAPRTase Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB317756
Artikelname: QPRT/QAPRTase Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB317756
Hersteller Artikelnummer: orb317756
Alternativnummer: BYT-ORB317756-50,BYT-ORB317756-100,BYT-ORB317756-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human QPRT/QAPRTase (21-120/297aa)
Konjugation: Unconjugated
Alternative Synonym: HEL-S-90n, QPRTase, NADC_BOVIN, QPRT, Quinolinate phosphoribosyltransferase [decarboxylating] (QAPRTase | QPRTase), 2.4.2.19, NADC_HUMAN,
QPRT/QAPRTase Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 31 kDa
Pubmed: 41338151
UniProt: Q15274
Puffer: Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
Formulierung: Liquid
Target-Kategorie: QPRT
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Quinolinate phosphoribosyltransferase, QPRT/QAPRTase) Polyclonal Antibody, Unconjugated (orb317756) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Sample: Lane 1: Mouse Liver tissue lysates, Lane 2: Mouse Large intestine tissue lysates, Lane 3: Mouse Kidney tissue lysates, Primary: Anti-QPRT/QAPRTase (orb317756) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 31 kDa, Observed band size: 28 kDa.
Sample: Liver (mouse) Lysate at 40 ug, Primary: Anti-QPRTQAPRTase (orb317756) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 31kD, Observed band size: 31kD.
Western blot analysis of mouse liver lysate using QPRT antibody.
Immunohistochemical staining of rat brain tissue using QPRT antibody.



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