Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunofluorescense analysis of Hela cell using BAG1 antibody (primary antibody dilution at: 1:10-50)
Western blot analysis of BAG1 Antibody (C-term) in MDA-MB231 cell line lysates (35 ug/lane). BAG1 (arrow) was detected using the purified Pab.
Confocal immunofluorescent analysis of BAG1 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of K562 cells using BAG1 Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram) FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human brain tissue reacted with BAG1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated.
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