Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None, Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Quelle:
Borrelia burgdorferi
Reinheit:
Crasp1 is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified Crasp1 and was estimated to be greater than 90% pure.
Formulierung:
Liquid (sterile filtered)
Application Verdünnung:
ELISA: User Optimized, WB: User Optimized
Anwendungsbeschreibung:
Biological Origin: Borrelia burgdorferi. Application Notes: Crasp1 is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect bands at 69.3 kDa for CRASP-1-MBP, (26.9 kDa for CRASP-1 and 42.4 kDa for MBP) in size corresponding to Crasp1 by Western blotting in the appropriate cell lysate or extract. Complement Regulator-Acquiring Surface Protein 1 was tested in SDS-page and western blot
Antigen-specific IgG2, IgA1, and ADCD partitioning between compartments differs significantly across disease phenotypes. (A) Fraction of samples with non-zero measurements for ADCD, IgG1, IgG2, IgG3, and IgA1 for refractory (dark blue) and responsive (green) patients in the serum (dashed line) and joint fluid (solid line) for each antigen. Significant differences in distribution of non-zero measurements between fluids as assessed by a Fishers exact test are denoted as *p < 0.05, **p < 0.01, ***p < 0.001 for refractory (dark blue) and responsive (green) samples after correction for multiple hypothesis testing via Benjamini-Hochburg. (B) Ratio of fraction of serum samples with non-zero measurements to fraction of joint fluid samples with non-zero measurements for ADCD, IgG1, IgG2, IgG3, and IgA1 for refractory (dark blue) and responsive (green) patients for each antigen. Significant differences in distributions of ratios between phenotypes are assessed by a Mann-Whitney nonparametric test, then corrected for multiple hypothesis testing via Benjamini-Hochburg. CRASP1, CRASP2, DbpA, DbpB, Arp37, flagellin, OspA, OspB, OspC, OspE, p27, p35, p39, VlsE: Biorbyt antigens.
Systems serology profiling with Borrelia-specific antigens reveals patient heterogeneity. The heatmap shows the Z-scored measurements for 12 features, across 16 antigens for both refractory and responsive patients, visualized with joint fluid measurements in the upper half of the heatmap and serum measurements in the lower half of the heatmap. Only antigens detected above background for at least 30% of samples were included for each measurement. Statistical significance was assessed using the Mann-Whitney nonparametric test, with p values then corrected for multiple hypothesis testing via Benjamini-Hochburg, *p < 0.05, **p < 0.01, ***p < 0.001, else not significant. CRASP1 (p/n orb345971), CRASP2 (p/n orb345972), DbpA (p/n orb345961), DbpB (p/n orb345969), Arp37 (p/n orb345962), flagellin (p/n orb345967), OspA (p/n orb345966), OspB (p/n orb345968), OspC (p/n orb345964), OspE (p/n orb345963), p27 (p/n orb345973), p35 (p/n orb345965), p39 (p/n orb345970), VlsE (p/n orb345974).
Western Blot results of Rabbit Anti-Crasp-1 Antibody. Lane 1: Crasp 1 protein (p/n orb345971). Lane 2: MBP. Load: 0.05 µl. Primary Antibody: Rabbit Anti-Crasp-1 Antibody (p/n orb344682) at 1.0 mg/ml overnight at 4C. Secondary Antibody: Goat anti-Rabbit (p/n orb347649) at 1:70000 for 30 min at RT. Blocking: BlockOut Buffer (p/n orb348644) for 30 min at RT. Expect: ~63.9kDa.
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