Preservative: None. Stabilizer: 10% (v/v) Glycerol, Buffer: 20 mM Tris pH 8, 300 mM NaCl with 10% glycerol
Quelle:
Human
Reinheit:
Recombinant protein mutant corresponds to amino acids 1 to 480 of mature human AKT1, Akt isoform 1. There are two point mutation of key activating residues, T308A and S473A. The recombinant protein contains a polyhistidine affinity tag at the amino terminus. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC.
Formulierung:
Liquid
Application Verdünnung:
WB: 50ng
Anwendungsbeschreibung:
Modification: Phosphorylated. Biological Origin: Human. Application Notes: Human AKT1 mutant recombinant protein has been tested in SDS-Page and western blot and is suitable as a control protein for immunoassays using antibodies targeting the T308 or S473 key phosphorylation sites. For western blot use at 50 ng or less. For other assays concentration is user optimized
SDS-PAGE of AKT1 (S473A, T308A) Human Recombinant Protein. Lane 1: AKT1 (S473A, T308A) unreduced. Lane 2: prestained MW markers. Lane 3: AKT1 (S473A, T308A), reduced. Load: 1 µg per lane. Predicted/Observed size: 56 kDa, ~56 kDa for AKT1 (S473A, T308A). Other band(s): none.
Western Blot of AKT1 (S473A, T308A) Human Recombinant Protein. Lane 1: SuperSignal MW markers. Lane2: AKT1. Load: 50 ng per lane. Primary antibody: AKT1 antibody at 1:1000 for 3.5 hours at room temperature. Secondary antibody: Peroxidase mouse secondary antibody at 1:20000 for 1 hour at room temperature. Block: Blocking Buffer for Fluorescent Western Blotting (orb348637), overnight at 4C. Predicted/Observed size: 56kDa, 56kDa for AKT1. Other band(s): none.
Western Blot of Rabbit AKT Antibodies. Lane 1: NIR MW protein ladder. Lane 2: AKT1, recombinant: orb346473. Lane 3: AKT1, phosphatase-treated: orb346472. Lane 4: AKT1, mutant T308A/S473A: orb346474. Lane 5: AKT2, recombinant: orb346475. Lane 6: AKT2, phosphatase-treated: orb346470. Lane 7: AKT3, recombinant: orb346476. Lane 8: AKT3, phosphatase-treated: orb346471. Load: 50 ng per lane. Blot A: orb345379 Anti-Akt pT308 used at 1:2270, Blot B: orb750474 Anti-Akt used 1:1000.
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