Application Notes: This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 52.0 kDa. The protein migrates as 75-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation
Captured Human IL-7 R alpha, Mouse IgG2a Fc Tag (Cat. No. orb402998) on CM5 chip via anti-mouse antibodies surface can bind ActiveMax Human IL-7, Tag Free (Cat. No. orb257603) with an affinity constant of 13.3 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
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