5-(3-Aminoallyl)-2-deoxyuridine-5-triphosphate, Sodium salt
Aminoallyl-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR, Nick Translation, Primer Extension, 3-End Labeling and Reverse Transcription. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The result-ing Amine-functionalized DNA/cDNA can subsequently be labeled via the classic Amine/NHS Ester reaction that offers the choice: to introduce a Biotin group (via NHS Ester of Biotin) for subse-quent purification tasks, to introduce fluorescent group (via NHS Ester of fluorescent dyes) for subsequent microscopic imaging.
Application Notes: Incorporation into DNA/cDNA by: PCR with Taqpolymerase in-house data, Nick Translation with DNAse I/DNA Polymerase I, Primer Extension with Klenow exo-, 3-End Labeling with Terminal deoxynucleotidyl Transferase (TdT), Reverse Transcription with MMLV Reverse Transcriptase. Spectroscopic Propertie: lambdamax 289 nm, epsilon 7.1 L mmol-1 cm-1 (Tris-HCl, pH 7.5)
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