5-Propargylamino-2-deoxycytidine-5-triphosphate, labeled with ATTO 647N, Triethylammonium salt
5-Propargylamino-dCTP-ATTO-647N is recommended for direct enzy-matic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine. Recommended Propargylamino-dCTP-ATTO-647N/dCTP ratio for Nick Translation: 30-50% Propargylamino-dCTP-ATTO-647N/70-50% dCTP.
