Phospho-GSK-3 Beta (Ser21+Ser29) Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB5356
- Bilder (6)
| Artikelname: | Phospho-GSK-3 Beta (Ser21+Ser29) Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB5356 |
| Hersteller Artikelnummer: | orb5356 |
| Alternativnummer: | BYT-ORB5356-50,BYT-ORB5356-100,BYT-ORB5356-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ICC, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated Synthesised phosphopeptide derived from human GSK-3 Beta around the phosphorylation site of Ser21+Ser29 QP(p-S)AFGSMKV(p-S)RD |
| Konjugation: | Unconjugated |
| Alternative Synonym: | GSK3B | GSK3 beta (p-S21/S29), p-GSK3 beta, phospho-GSK3 beta, 7330414F15Rik, 8430431H08Rik, GSK-3, GSK-3beta, GSK3, GSK3B_HUMAN, GSK3B, GSK-3 beta, Serine/threonine-protein kinase GSK3B, 2.7.11.26, GSK3B_MOUSE, |
| Phospho-GSK-3 Beta (Ser21+Ser29) Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 46 kDa |
| UniProt: | P49841 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | GSK3B |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500 |
| Anwendungsbeschreibung: | Modification: Phosphorylated |
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A431 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (phospho-GSK-3 Beta (Ser21+Ser29)) polyclonal Antibody, Unconjugated (orb5356) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (phospho-GSK-3 Beta (Ser21+Ser29)) Polyclonal Antibody, Unconjugated (orb5356) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (phospho-GSK-3 Beta (Ser21+Ser29)) Polyclonal Antibody, Unconjugated (orb5356) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GSK-3 Beta (Ser21+Ser29)) Polyclonal Antibody, Unconjugated (orb5356) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Muscle (Mouse) Lysate at 40 ug, Lane 2: Liver (Mouse) Lysate at 40 ug, Lane 3: Cerebrum (Mouse) Lysate at 40 ug, Lane 4: Pancreas (Mouse) Lysate at 40 ug, Lane 5: Thymus (Mouse) Lysate at 40 ug, Lane 6: Muscle (Rat) Lysate at 40 ug, Lane 7: Cerebrum (Rat) Lysate at 40 ug, Lane 8: Pancreas (Rat) Lysate at 40 ug, Lane 9: Thymus (Rat) Lysate at 40 ug, Lane 10: Hela (Human) Cell Lysate at 30 ug, Lane 11: MCF-7 (Human) Cell Lysate at 30 ug, Lane 12: A431 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-GSK-3 Beta (Ser21+Ser29) (orb5356) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kD, Observed band size: 47 kD. |
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Western blot analysis using Anti-phospho-GSK-3 Beta (Ser21+Ser29) antibody at 1:1000 dilution. |
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