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Phospho-GSK-3 Beta (Ser9) Recombinant Rabbit Monoclonal Antibody, Clone: [7A4], Unconjugated

Phospho-GSK-3 Beta (Ser9) Recombinant Rabbit Monoclonal Antibody, Clone: [7A4], Unconjugated

Artikelnummer: BYT-ORB544567

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Artikelname:	Phospho-GSK-3 Beta (Ser9) Recombinant Rabbit Monoclonal Antibody, Clone: [7A4], Unconjugated
Artikelnummer:	BYT-ORB544567
Hersteller Artikelnummer:	orb544567
Alternativnummer:	BYT-ORB544567-50,BYT-ORB544567-100
Hersteller:	Biorbyt
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	ICC, IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität:	Human
Immunogen:	KLH conjugated Synthesised phosphopeptide derived from human GSK-3 Beta around the phosphorylation site of Ser9 TT(p-S)FA
Konjugation:	Unconjugated
Alternative Synonym:	GSK3B \| GSK3 beta (p-S9), p-GSK3 beta, phospho-GSK3 beta, 7330414F15Rik, 8430431H08Rik, GSK-3, GSK-3beta, GSK3, GSK3B_HUMAN, GSK3B, GSK-3 beta, Serine/threonine-protein kinase GSK3B, 2.7.11.26, GSK3B_MOUSE,

Phospho-GSK-3 Beta (Ser9) Recombinant Rabbit Monoclonal Antibody

Klonalität:	Recombinant
Konzentration:	1mg/ml
Klon-Bezeichnung:	[7A4]
Molekulargewicht:	47 kDa
UniProt:	P49841
Puffer:	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung:	Liquid
Target-Kategorie:	GSK3B

Application Verdünnung:	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500
Anwendungsbeschreibung:	Modification: Phosphorylated

	Paraformaldehyde-fixed, paraffin embedded (human breast), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-GSK-3 Beta (Ser9)) Polyclonal Antibody, Unconjugated (orb544567) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-GSK-3 Beta (Ser9)) Polyclonal Antibody, Unconjugated (orb544567) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-GSK-3 Beta (Ser9)) Polyclonal Antibody, Unconjugated (orb544567) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (human pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (phospho-GSK-3 Beta (Ser9)) Monoclonal Antibody, Unconjugated (orb544567) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
	Sample: MDA-MB-231 (Human) Cell Lysate at 30 ug, 293T (Human) Cell Lysate at 30 ug, SW480 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-GSK-3 Beta (Ser9) (orb544567) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kD, Observed band size: 47 kD.
	IHC-P analysis of human breast using phospho-GSK-3 Beta Ser9 Polyclonal Antibody at 1:200 dilution.

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