Smad2 Recombinant Rabbit Monoclonal Antibody, Clone: [9A3], Unconjugated
Artikelnummer:
BYT-ORB544569
- Bilder (6)
| Artikelname: | Smad2 Recombinant Rabbit Monoclonal Antibody, Clone: [9A3], Unconjugated |
| Artikelnummer: | BYT-ORB544569 |
| Hersteller Artikelnummer: | orb544569 |
| Alternativnummer: | BYT-ORB544569-50,BYT-ORB544569-100 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | FC, ICC, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | A synthesized peptide derived from human SMAD2 (230-270aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | CHTD8, JV18, JV18-1, LDS6, MADH2, MADR2, hMAD-2, hSMAD2, 7120426M23Rik, Smad-2, mMad2, SMAD2_HUMAN, SMAD2, MAD homolog 2, Mothers against DPP homolog 2, Mad-related protein 2 (hMAD-2), SMAD family member 2 (SMAD 2 | Smad2 | hSMAD2), SMAD2_MOUSE, Mad-related protein 2 (mMad2), SMAD family member 2 (SMAD 2 | Smad2), SMAD2_RAT, Mad-related protein 2, |
| Smad2 Recombinant Rabbit Monoclonal Antibody |
| Klonalität: | Recombinant |
| Konzentration: | 1mg/ml |
| Klon-Bezeichnung: | [9A3] |
| Molekulargewicht: | 52 kDa |
| UniProt: | Q15796 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | SMAD2 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:50-200, IF=1:50-200, Flow-Cyt=1ug/Test |
|
|
A549 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Smad2) monoclonal Antibody, Unconjugated (orb544569) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
|
|
Blank control: A431. Primary Antibody (green line): Rabbit Anti-Smad2 antibody (orb544569), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
|
|
Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Smad2) Polyclonal Antibody, Unconjugated (orb544569) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Smad2) Polyclonal Antibody, Unconjugated (orb544569) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Smad2) Polyclonal Antibody, Unconjugated (orb544569) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Sample: Testis (Mouse) Lysate at 40 ug, A431 (Human) Cell Lysate at 30 ug, HepG2 (Human) Cell Lysate at 30 ug, HL60 (Human) Cell Lysate at 30 ug, Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-Smad2 (orb544569) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kD, Observed band size: 53 kD. |
Produktgarantie und fachkundiger Support
