Phospho-IKB alpha (Tyr42) Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB5520
Artikelname: Phospho-IKB alpha (Tyr42) Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB5520
Hersteller Artikelnummer: orb5520
Alternativnummer: BYT-ORB5520-50,BYT-ORB5520-100,BYT-ORB5520-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: FC, IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse
Immunogen: KLH conjugated Synthesised phosphopeptide derived from human IKB alpha around the phosphorylation site of Tyr42 EE(p-Y)EQ
Konjugation: Unconjugated
Alternative Synonym: NFKBIA | IKB alpha (p-Y42), p-IKB alpha, phospho-IKB alpha, EDAID2, IKBA, MAD-3, NFKBI, IKBA_HUMAN, NFKBIA, I-kappa-B-alpha (IkB-alpha | IkappaBalpha), Major histocompatibility complex enhancer-binding protein MAD3, MAD3,
Phospho-IKB alpha (Tyr42) Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 35 kDa
UniProt: P25963
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: NFKBIA
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test
Anwendungsbeschreibung: Modification: Phosphorylated
Blank control (blue line): Hela (fixed with 70% methanol (Overnight at 4C) and then permeabilized with 90% ice-cold methanol for 20 min at -20C). Primary Antibody (green line): Rabbit Anti-phospho-IKB alpha (Tyr42) antibody (orb5520), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.
Blank control (blue line): Jurkat (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-phospho-IKB alpha (Tyr42) antibody (orb5520), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (p-IKB alpha (Tyr42)) Polyclonal Antibody, Unconjugated (orb5520) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: HepG2 (Human) Cell Lysate at 30 ug, Lane 2: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-IKB alpha (Tyr42) (orb5520) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 39 kD, Observed band size: 37 kD.
Sample: Raji (Human) Lysate at 40 ug, Primary: Anti-phospho-IKB alpha (Tyr42) (orb5520) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 35 kD, Observed band size: 35 kD.
Sample: Thymus (Mouse) Lysate at 40 ug, Primary: Anti-phospho-IKB alpha (Tyr42) (orb5520) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 35 kD, Observed band size: 35 kD.



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