YAP1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB5629
- Bilder (7)
| Artikelname: | YAP1 Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB5629 |
| Hersteller Artikelnummer: | orb5629 |
| Alternativnummer: | BYT-ORB5629-100,BYT-ORB5629-200,BYT-ORB5629-50 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ICC, IF, IHC-Fr, IHC-P |
| Spezies Reaktivität: | Human |
| Immunogen: | KLH conjugated synthetic peptide derived from human YAP1 (131-330/504aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | COB1, YAP, YAP-1, YAP2, YAP65, YKI, YAP1_HUMAN, YAP1, Yes-associated protein 1, Protein yorkie homolog, Yes-associated protein YAP65 homolog, |
| YAP1 Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 55 kDa |
| UniProt: | P46937 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | YAP1 |
| Application Verdünnung: | IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500 |
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HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (YAP1) polyclonal Antibody, Unconjugated (orb5629) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (YAP1) polyclonal Antibody, Unconjugated (orb5629) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Tissue/Cell: human breast cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-YAP1 Polyclonal Antibody, Unconjugated (orb5629) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: human colon cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-YAP1 Polyclonal Antibody, Unconjugated (orb5629) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: human gastric carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-YAP1 Polyclonal Antibody, Unconjugated (orb5629) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: human hepatoma tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-YAP1 Polyclonal Antibody, Unconjugated (orb5629) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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IHC-P of human breast cancer tissue using YAP1 antibody (dilution at 1:200) |
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